Fig. 5: Nuclear p85β stabilizes EZH1/EZH2 and enhances H3K27me3 to regulate gene expression.
From: Nuclear translocation of p85β promotes tumorigenesis of PIK3CA helical domain mutant cancer
a A heat map of RNA-seq analyses of DLD1 parental cells and p85β NLS mutant (p85βKR-AA KI) cells. b–e Nuclear p85β stabilizes EZH1 and EZH2 and increases histone H3K27 methylation in PIK3CA helical domain mutant cells, but not in PIK3CA WT or H1047R mutant cells. H3K27me3, EZH1, and EZH2 levels were evaluated by Western blot analyses in indicated cell lines. Similar results were reproduced three times. f, g ChIP-seq analyses of H3K27me3 in DLD1 parental cells and p85β NLS mutant cells. A heat map is shown in (f), and a Venn diagram is shown in (g).
