Fig. 3: Identification of putative NEDD4 targets. | Nature Communications

Fig. 3: Identification of putative NEDD4 targets.

From: Global ubiquitinome profiling identifies NEDD4 as a regulator of Profilin 1 and actin remodelling in neural crest cells

Fig. 3

a Tabulated values for the number of ubiquitin (ub) remnant enriched peptides satisfying criteria of being affected by Nedd4 knockdown, the number of corresponding protein groups and a breakdown of abundance changes for proteins in either group. A breakdown of these values across the entire dataset (for all ubiquitin remnant peptides with cognate proteins in the pre-enrichment dataset) is shown on the last line. b 2D plot of log2 H/L ratios for all ubiquitin remnant enriched (K-ε-GG) peptides (y-axis) versus the log2 H/L ratio of their cognate protein (x-axis). Ubiquitin remnant enriched peptides with over 3-fold decrease compared to matched total protein are shown in green. Ubiquitin remnant peptides over 3-fold increase compared to matched total protein are shown in magenta. Ubiquitination events on PFN1 are shown in red, and KIF16B in purple. c Proteins with peptides containing over 3-fold reduced ubiquitination that are annotated in the actin cytoskeleton pathway. Only the top-ranked site for each protein is shown with corresponding lysine residue.

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