Fig. 1: Development of NanoTACs; a NanoLuc-targeting degrader system. | Nature Communications

Fig. 1: Development of NanoTACs; a NanoLuc-targeting degrader system.

From: Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms

Fig. 1

a Schematic depicting the tTPD systems. FKBP12F36V, NanoLuc or Halo epitope tags are fused to proteins of interest (POI) and tag-targeting heterobifunctional degrader compounds are employed to hijack cullin-RING ligase (CRL) complexes to trigger proteasomal degradation of the tagged POI. b Comparison of tools and properties of each tag for tag-targeted protein degradation. NT not tested. Asterisk (*) see discussion for further information. c Schematic depicting the NanoLuc-CRBN tTPD system. d Chemical structure of the heterobifunctional NanoLuc-targeting PROTAC; NanoTAC4 (NC4).

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