Table 1 Mutagenic analysis of dephosphorylation by core catalytic domain AtPFA-DSP149–215.

From: A structural exposé of noncanonical molecular reactivity within the protein tyrosine phosphatase WPD loop

 

WT

C150S

H155D

H155E

H155L

T157A

D191A

Specific activity nmol min−1 mg−1

674 ± 60 (7)

0.15 ± 0.03 (6)

35.1 ± 2.7 (7)

15.8 ± 1.4 (9)

12.6 ± 0.1 (6)

15.1 ± 0.8 (9)

46.2 ± 1.5 (7)

Relative activity %

100

0.02

5.2

2.3

1.9

2.2

6.9

  1. Data are means and standard errors; the numbers of independent experiments (see Methods) are provided in parentheses. Experiments were performed with 10 µM 5-InsP7; a colorimetric assay was used to determine Pi release, except for the C150S mutant, for which 5-[3H]InsP7 hydrolysis was directly monitored by HPLC (see Methods and Supplementary Fig. 3). Source data are provided as a Source Data file.
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