Fig. 5: Mutations that alter growth and body size do not uncouple growth and development.

a Scatter plot of the rate of development α  (=1/larval stage duration) vs. the volume growth rate for indicated mutants and larval stages. Error bars: SEM between day-to-day repeats. Thick grey line indicates proportional scaling corresponding to the volume fold change of the wild type. Dotted lines indicate deviation in volume fold change for a given deviation from proportionality. Region above the thick line corresponds to an increase in volume fold change, region below the thick line to a decrease in volume fold change compared to the wild type. b Box plot of volumes at birth and larval moults for indicated mutant strains. Colour scheme as indicated in legend of (a). Individual points and box plots indicate average volume of each day-to-day repeat. For p-values calculated by two-sided ranksum test of day-to-day repeats between mutant and wild type see Supplementary Table 1. c Same as (b), but for body length. d Same as (a), but for coefficient of variation of the growth rate. e Correlation between growth rate and the larval stage duration among individuals for different mutant backgrounds shown as deviation from the mean. Colours correspond to legend shown in (a). Solid lines show a robust linear regression to data. Dotted lines are 95% confidence interval of the fit. Scatter plot of individual data points is omitted for clarity of presentation (see Supplementary Fig. 8). a–d Boxplots: central line: median, box: interquartile ranges (IQR), whisker: ranges except extreme outliers (>1.5*IQR). Number of animals (n) for L1 to L4 from number of independent experiments (m): wild type: n = 639, 1,142, 1,144, 1,095; m = 10. eat-2: n = 270, 439, 440, 417; m = 3. db++: n = 433, 643, 650, 640; m = 2. lon-1: n = 450, 712, 716, 701; m = 7. raga-1: n = 347, 504, 501, 483; m = 3. lin-14: n = 288, 410, 387, n/a. m = 2.