Fig. 3: The crown depth critically affects the Nanocrown electrode performance.
From: Nanocrown electrodes for parallel and robust intracellular recording of cardiomyocytes
A Electroporation success rates for Nanopillar electrodes (blue) and Nanocrown electrodes with 450 nm depth (green) and Nanocrown electrodes with 180 nm depth (Orange). Data are presented as mean values ± SD. B The initial amplitudes of intracellular action potentials (iAPs) measured by Nanopillar electrodes (blue), Nanocrown electrodes with 450 nm depth (green) and Nanocrown electrodes with 180 nm depth (Orange). C Percent of cells that maintained intracellular access at the end of the recording (~240 s after electroporation). D schematic representation of the interface of the cell membrane and the nanocrown electrodes of various depths (450 nm and 180 nm). E An iAP recording trace with 180-nm-deep nanocrown shows an iAP amplitude of ~63 mV at t = 1 min, ~30 mV at t = 1 h and ~16 mV at t = 1.5 h. F The average iAP amplitude of many cells that are recorded daily remains stable over the 12-day period, indicating that daily-repeated electroporation and resealing cycles do not degrade the recording quality. n = 16 cells from three independent cell cultures from day-on-device 38 (DOD38) to DOD49. Six more cells were added on DOD41 for a total of n = 22 cells recorded from DOD41 to DOD49. Data are presented as mean values ± SD.
