Fig. 4: Simultaneous Patch clamp and nanoelectrode array (NEA) recordings verify the accuracy of NEA-recorded intracellular action potentials (iAPs).
From: Nanocrown electrodes for parallel and robust intracellular recording of cardiomyocytes
A A schematic representation of simultaneous patch and NEA recording from the same cell. B A brightfield image showing a patch pipette approaching a cell on the nanopillar electrode from the left. C Simultaneous recording traces by patch clamp (orange) and NEA (blue) from the same cell. The two traces are aligned in time with t = 0 for electroporation. D Zoomed-in time windows of NEA- and Patch- recordings before electroporation (left) and after electroporation (right). Before electroporation, NEA measured eAP spikes while patch clamp measured iAPs with high amplitudes. After electroporation, both NEA and patch measured iAPs. E Three pairs of NEA- and Patch-recorded iAPs are amplitude normalized and overlaid together. The overlays show identical waveforms. F Analyzed features of iAP waveforms: APD50, APD90, cycle time and diastolic interval and amplitudes. G Deviations of iAP features between NEA- and patch-recorded iAPs for ten independent cells. H Averaged values of deviations of iAP features between NEAs and Patch for N = 10 cells. Data are presented as mean values ± SD.
