Fig. 5: Comparison of APD features in simultaneous Patch clamp and nanoelectrode arrays (NEA) recordings.
From: Nanocrown electrodes for parallel and robust intracellular recording of cardiomyocytes
A Simultaneous recording traces by patch clamp (orange) and NEA (blue) from the same cell. The two traces are aligned in time with t = 0 for electroporation. B Zoomed-in time windows of NEA- and Patch- recordings directly before and after electroporation (Interval 1), 80 s after electroporation (Interval 2), and 425 s after electroporation (Interval 3). The significant points on each iAP include spiking (red stars), starting (green stars), maximum (blue stars), and the minimum (cyan stars) points which are determined automatically by our in-house software. C Overlay of time-dependant changes in action potential duration (APD) features calculated using the Patch (Orange) or NEA (blue) recordings including cycle time (s), APD50 (s), APD90 (s), diastolic interval (s), amplitude (mV) and spiking velocity- dV/dt (mV/s). D Comparison of iAP amplitudes between patch (top-orange) and NEA (middle-blue) recordings at four time intervals after electroporation (80 s, 300 s, 430 s, and 530 s). As the membrane pores sealed over time, the amplitude of NEA-recorded iAPs decreased. Nevertheless, the scaled NEA and Patch recordings (Bottom row) largely overlap at various time intervals. E Time dependent deviations of AP waveforms of patch and NEA recordings. F Time dependent deviations of APD90 calculated from patch or NEA recordings.
