Fig. 7: In-situ iAP recording using miniaturized petri-dish-like recording stage.
From: Nanocrown electrodes for parallel and robust intracellular recording of cardiomyocytes
A Photographs of a Nanocrown device bound to a printed circuit board and integrated with a bottomless 35-mm petri dish. When placed on the recording stage, the electrode pads at the bottom are in contact to pogo pins inside the stage. B A plastic 3D printed well (white) is assembled on top of the petri-dish NEA for cell culture. C The petri dish NEA is situated in a Cyion recording stage. The recording stage is housed inside a CO2 incubator for in-situ recording. D The measured cycle time of beating human pluripotent stem-cell-derived cardiomyocytes (hPSC-CMs) gradually increases over 400 s on the MCS recording stage (blue trace, outside the incubator), while the measured cycle time remains stable when using the miniaturized Cyion stage (orange trace, inside the incubator).
