Fig. 1: Architecture of the CaV3.3 channel.
From: Structure, gating, and pharmacology of human CaV3.3 channel
a The activation (right) and inactivation (left) properties of the truncated CaV3.3EM and full-length wild type (WT) CaV3.3WT. Normalized conductance (G/Gmax) for the activation properties is applied by a held at −100 mV and then a series of 200 ms voltage steps from −100 mV to +20 mV in 10 mV increments. The ion current (I/Imax) for inactivation (left) properties are assessed with a 3.6 s holding voltages ranging from −110 mV to 0 mV (10 mV increments) followed by a 150 ms test pulse at −20 mV. N cells were analyzed (WT, n = 7; EM, n = 8). Data are presented as mean values +/– SEM. Source data are provided as a Source Data file. b The overall structure of the CaV3.3apo. The domains of CaV3.3 are colored as DI in purple, DII in green, DIII in blue, and DIV in salmon. A cation ion in the selectivity filter vestibule was presented as a gray sphere. c The ion permeation path in the pore domain. The selectivity filter and S5-S6 helices are shown in cartoon and viewed in parallel to the membrane plane. The ion-conducting pathway was calculated by the program HOLE and illustrated by gray dots in the left panel. The selectivity filter ring of four negatively charged residues from the four domains is shown in sticks in the upper, and the intracellular gate formed by four S6 helix viewed from extracellular side was shown in the lower of the right panel, respectively.
