Fig. 3: Differential responses of the iM0φ, iM1φ and iM2φ to SARS-CoV-2 infection. | Nature Communications

Fig. 3: Differential responses of the iM0φ, iM1φ and iM2φ to SARS-CoV-2 infection.

From: Differential effects of macrophage subtypes on SARS-CoV-2 infection in a human pluripotent stem cell-derived model

Fig. 3

a Schematic of the experimental flowchart of SARS-CoV-2 infection of the co-culture systems. b–e IF staining of the co-cultures of iLungs with 293 T cells (b), iM0φs (c), iM1φs (d), and iM2φs (e) infected with SARS-CoV-2 at 24 hpi (MOI = 0.1) and mock samples, using antibodies detecting SARS-CoV-2 nucleocapsid protein (SARS-CoV-2), CD68 (M0φ), CD80 (M1φ), and CD206 (M2φ). iLung cells expressed GFP. Scale bar = 100 µm. f Quantification of infected lung cells in the co-cultures at 24 hpi by FACS using antibodies against SARS-2 N and S protein. n  =  3 independent experiments. Data are presented as mean values ± SD. p values were calculated by one-way ANOVA with Tukey’s multiple comparison test.

Back to article page