Fig. 1: Identification of cell populations in murine esophageal epithelium.

a Schematic overview of experimental design. b Expression z-scores for the top 5 upregulated genes in each cluster. Red indicates enrichment while blue indicates inhibition. c Seurat’s Uniform Manifold Approximation and Projection (UMAP) was used to identify distinct cell populations within the epithelial dataset. Eleven epithelial cell populations were identified. d Genes identified as primary contributors to UMAP based on their loadings are listed and their expression z-scores in cells across the epithelial dataset are shown. Red indicates enrichment while blue indicates inhibition. e, f Log1p normalized expression of the basal markers Krt14 and Krt5 (e) and superficial markers Fabp5, Krt13, Krt4, and Krtdap (f) across the epithelial dataset is shown. Purple indicates enrichment. g Representative image of RNA fluorescence in situ hybridization to visualize Krt5 and Krtdap in murine esophageal epithelium in situ (n = 6 animals). Scale bar, 50 μm.