Fig. 7: Pseudotemporal projection of cell populations and proposed model of cell fate in in murine esophageal epithelium.

a Monocle 3’s Uniform Manifold Approximation and Projection (UMAP) visualization of murine epithelial cells in the dataset. Each cell is colored by its inferred pseudotime value with dark purple representing the earliest cells and bright yellow representing the latest cells in the trajectory. b Clusters identified in in the integrated analysis of the epithelium labeled in the pseudotime projection. c Expression of genes associated with each phase of the cell cycle were labeled in pseudotime projection. d A proposed model of cell fate trajectories in murine esophageal epithelium. e Modules of co-expressed genes and their expression in the 11 cell populations identified in murine esophageal epithelium. A positive value (red) indicates activation while a negative value (blue) indicates inhibition of the respective pathway. Module 18 is highlighted because of its enrichment in basal clusters 4, 5, and 6, which comprise the proposed alternative cell fate in murine esophageal epithelium. Within Module 18, relative gene expression level is indicated from lowest (dark blue) to highest (light green). Significantly enriched pathways as identified by Ingenuity pathway analysis are listed along with genes in two pathways of interest, Regulation of epithelial mesenchymal transition (EMT) in Development Pathways and Wnt/β-catenin Signaling.