Fig. 5: DENR depletion stalls ribosomes on uORFs.
From: DENR controls JAK2 translation to induce PD-L1 expression for tumor immune evasion
a DENR was immunoprecipitated with the anti-DENR antibody, the experiment was repeated independently three times with similar results. b RIP analysis of DENR association with mRNAs in MC38 cells. DENR-bound mRNAs were detected by RT-qPCR (n = 3 biologically independent samples). Data are presented as mean values ± SD, unpaired two-tailed Student’s t-test: * p < 0.05, ** p < 0.01. p values from left to right: 0.0144, 0.0074, and 0.0026. c Representative traces of polyribosome profiles obtained from control or DENR KO MC38 cells. The experiment was repeated independently three times with similar results. d RT-qPCR analysis of Jak1 mRNA levels in each fraction obtained from control or DENR KO MC38 cells (n = 3 independent samples). Results are representative of two biological replicates. Three technical replicates are shown. Data are presented as mean values ± SD, unpaired two-tailed Student’s t-test: ns, not significant. p values from left to right: 0.1344, 0.1233, and 0.9869. e RT-qPCR analysis of Jak2 mRNA levels in each fraction obtained from control or DENR KO MC38 cells (n = 3 independent samples). Results are representative of two biological replicates. Three technical replicates are shown. Data are presented as mean values ± SD, unpaired two-tailed Student’s t-test: ** p < 0.01, *** p < 0.001. p values from left to right: 0.0015, 0.0032, 0.0086, <0.0001, 0.0006. f 40S and 80S ribosome occupancy of JAK2 mRNA transcripts in DENR KO versus WT Hela cells. Source data are provided as a Source Data file.
