Fig. 6: DENR depletion reduces tumor growth and PD-L1 expression in vivo.
From: DENR controls JAK2 translation to induce PD-L1 expression for tumor immune evasion
a, b Control and DENR KO MC38 cells stably expressing tdTomato or EGFP were mixed at a 1:1 ratio and administered subcutaneously into 6–8 weeks old female WT C57BL/6 mice (n = 5 mice). The composition of EGFP and tdTomato-positive cells in tumors was analyzed on day 8 or 12 by FACS. Data are presented as single values and are representative of three independent experiments. Paired two-tailed Student’s t-test: ns, no significant, ** p < 0.01. p values from left to right: 0.1078, 0.0032. c Cell surface PD-L1 on control or DENR KO MC38 cells in tumors on day 8 as shown in a was analyzed by FACS (n = 5 tumors). Data are presented as single values and are representative of three independent experiments with similar results. Paired two-tailed Student’s t-test: ** p < 0.01. p = 0.0017. d, e Female WT C57BL/6 mice (6–8 weeks) were given 5 × 105 control or DENR KO tumor cells, and tumors were measured every 1–2 days (n = 5 mice). Tumor growth is representative of three independent experiments with similar results. Data are presented as mean values ± SD, two-way ANOVA test: *** p < 0.001. p values from top to bottom: < 0.0001, <0.0001. f, g FACS analysis of intratumor CD4+ and CD8+ T cells in B16/F10 tumors (n = 5 tumors) as shown in (d). Data are presented as mean values ± SD and are representative of three independent experiments with similar results. Unpaired two-tailed Student’s t-test: ns, no significant, ** p < 0.01. p values from left to right: 0.1712, 0.0030. Source data are provided as a Source Data file.
