Fig. 2: The dirigent family gene Zm00001d033942 is the candidate of ZmSTL1.
From: A dirigent family protein confers variation of Casparian strip thickness and salt tolerance in maize
a BSA-based analysis mapped ZmSTL1 to a 20-Mb region on Chromosome 1 (see methods). (b) High-resolution linkage assay mapped ZmSTL1 to a 110-kb region (see “Methods”). The graphical genotypes of five important recombinants were shown. Green, gray and yellow bars represented regions homozygous for 3H-2, regions with undetermined genotypes, and regions homozygous for CIMBL45, respectively. The two genes within the 110-kb candidate region were shown. c–f The appearances (c, d) and SPAD values (e, f) of Zm00001d033942crispr and wild-type plants grown under control and salt (100 mM NaCl) conditions with indicated relative humidity (RH). g Alignment of the amino acid sequences of Zm00001d0339423H-2 and Zm00001d033942CIMBL45. The frameshifted sequence of Zm00001d033942CIMBL45 was highlighted in blue. The results in (e) and (f) were means ± s.d. of three independent experiments. Statistical significance was determined by a two-sided t-test. Bars in (c) and (d), 10 cm. Source data are provided as a Source Data file.
