Fig. 3: Phylogenetic analysis, expression pattern and the subcellular localization of Zm00001d033942/ZmESBL.
From: A dirigent family protein confers variation of Casparian strip thickness and salt tolerance in maize
a The phylogenetic tree for maize, rice, soybean and Arabidopsis DIR proteins. b The transcript levels of ZmESBL in root and shoot tissues. c The transcript levels of ZmESBL in indicated samples. The data was downloaded from MaizeGDB a, 6–7 internode; b, 7–8 internode; c, leaf zone 1; d, leaf zone 3; e, mature leaf 8; f, root meristem zone; g, root elongation zone; h, root cortex; i, primary root; j, secondary root. d The in-situ RT-PCR assay. The dark blue signal indicated the presence of ZmESBL transcripts. No reverse transcription (No RT) and the 18S rRNA (18S) provided controls. e The subcellular location of GFP-ZmESBL protein in the Arabidopsis plants transformed with pAtESB1-GFP-ZmESBL. The red fluorescence indicated the basic fuchsin staining of lignin. The results in (b) were means ± s.d. of three independent experiments. The images in (d) and (e) were representative of three independent repeats with similar results. Bars in (d) and (e), 20 μm. Source data are provided as a Source Data file.
