Fig. 5: In vitro ROS generation of compound 1. | Nature Communications

Fig. 5: In vitro ROS generation of compound 1.

From: An unexpected strategy to alleviate hypoxia limitation of photodynamic therapy by biotinylation of photosensitizers

Fig. 5

CLSM images of MCF-7 cells stained with DCFH-DA (a), compound 1 (10 μM) (b), and DCFH-DA + compound 1 with different light doses under (c, d) normoxic (21% O2) and (e) hypoxic (1% O2) conditions. Ex: 488 nm, Em: 500–550 nm. Scale bar: 30 μm. CLSM images of MCF-7 cells stained with compound 1 (10 μM) under normoxic (21% O2, f) and hypoxic (1% O2, g) conditions using DHE as the intracellular O2−• fluorescence indicator before and after white-light irradiation (“+” represents with irradiation, “−” represents without irradiation, white light 20 mW/cm2, 10 min). Ex: 488 nm, Em: 590–630 nm. Scale bar: 30 μm. Each experiment was repeated three times independently, with similar results.

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