Fig. 1: The M. tuberculosis Δrv0455c mutant is hypersensitive to exogenous siderophores. | Nature Communications

Fig. 1: The M. tuberculosis Δrv0455c mutant is hypersensitive to exogenous siderophores.

From: A periplasmic cinched protein is required for siderophore secretion and virulence of Mycobacterium tuberculosis

Fig. 1

a–d The indicated M. tuberculosis strains were grown in low-iron 7H9 medium for 5 days to deplete intracellular iron before the growth assays. The initial OD600 of all cultures was 0.01. Growth curves of M. tuberculosis mc26230 (wt), Δrv0455c (M. tuberculosis ML2203), Δrv0455c::rv0455c (M. tuberculosis ML2205), ΔmmpS4/S5 (M. tuberculosis ML859) and ΔmbtD (M. tuberculosis ML1600) in (a) self-made low-iron (<0.1 μM Fe3+) 7H9 medium supplemented with (b) 10 μM hemin, (c) 10 μM hemin plus 100 nM Fe-mycobactin (Fe-MBT), and (d) 10 μM hemin plus 1 μM Fe-carboxymycobactin (Fe-cMBT), respectively. Error bars represent standard deviations from the mean results of biological triplicates (n = 3). e, f The viability of M. tuberculosis wt, ΔmmpS4/S5, Δrv0455c, or Δrv0455c::rv0455c was measured using the Microplate alamarBlue assay. Cells treated with increasing concentrations of (e) Fe-mycobactin (Fe-MBT) or (f) Fe-carboxymycobactin (Fe-cMBT). Cells were grown in 7H9 medium supplemented with 20 μM hemin. Error bars represent standard deviations from the mean values of biological triplicates (n = 3). Source data are provided in the Source Data file.

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