Fig. 5: An elongated interprotomer is critical for VFT coordination.
From: Mechanism of sensitivity modulation in the calcium-sensing receptor via electrostatic tuning
a Surface representation of CaSR (left, PDB 5K5S) and mGluR1 (right, PDB 1ISR) showing contacts made between monomers in the inactive structure only (red), active structure only (green), or both (yellow). b Multiple sequence alignment of human CaSR and mGluRs. CaSR is used as reference for residue numbering. Sequence of interprotomer loop indicated by red dashed line. c Surface and ribbon representation of CaSR (left, PDB 5K5S), and mGluR1 (right, PDB 1ISR) with interprotomer loop colored red. d smFRET population histogram for P55L in the presence of 3 mM EDTA, 5 mM l-Trp, 10 mM Ca2+ and 5 mM l-Trp, or 40 mM Ca2+ and 5 mM l-Trp. For reference, dashed lines indicate centers of wild-type distributions for EDTA (gray) and 10 mM Ca2+ + 5 mM l-Trp (purple). Data represent mean ± s.e.m. of n = 3 independent biological replicates.
