Fig. 6: TGS1 regulates EXO1 activity at telomeres.

a Representative images of DNA-FISH experiment using a telomere G-rich strand-specific probe on H1299 cells transfected, as described, for 10 days. b Quantification of telomere G-rich strand fluorescence intensity of cells in a. c Representative images of DNA-FISH experiment using telomere C-rich strand-specific probe on H1299 cells transfected, as indicated, for 10 days. d Quantification of telomere C-rich strand fluorescence intensity of cells in c. e Representative images of telomere DNA-FISH combined with immunofluorescence using anti-Coilin antibody in H1299 cells transfected with indicated siRNAs for 10 days. f Quantification of experiment shown in e. g Representative images of telomere DNA-FISH combined with immunofluorescence using anti-PML antibody in H1299 cells transfected with indicated siRNAs for 10 days. h Quantification of experiment shown in g. i Representative images of telomere DNA-FISH combined with immunofluorescence using anti-PML antibody in H1299 cells treated as indicated. j Quantification of experiment shown in i. k Representative images of DNA-FISH of G-rich telomere strands on H1299 cells treated as indicated. l Telomere fluorescence-intensity analysis of cells shown in k. m Representative images of DNA-FISH of C-rich telomere strands on H1299 cells treated as indicated for 10 days. n Telomere fluorescence- intensity analysis of cells shown in m. o Representative images of immunofluorescence analysis using anti-TRF1 and anti-POLD3-specific antibodies. H1299 cells were treated as indicated. p Quantification of experiment shown in o. In Fig. b, d, l, n violin-plot diagrams are shown: middle line represents median arbitrary fluorescence units (a.f.u.), dotted lines mark the highest and lowest quartile. For quantifications in f, h, j, l, p mean values are indicated; whiskers indicate standard deviation. Bars indicate mean values. In Fig. b, d, l, n N = number of analyzed nuclei, n = telomere-repeat signals. In Fig. f, h, j, p N = number of independent experiments. n = number of analyzed nuclei. Arrowheads indicate colocalization events. Red and green, epitopes detected by immunofluorescence; blue, DAPI-stained nuclei. Scale bar, 1 µm. A two-tailed Student’s t-test was used to calculate statistical significance; p-values are shown.