Fig. 1: Overview of the experimental design.
From: Mutational meltdown of putative microbial altruists in Streptomyces coelicolor colonies
a Initial and final frequency of three T0 strains from different lineages during the 5-day competition with the WT ancestor. The dashed line shows the expectation if initial and final frequencies are equal, as seen for the strain from the WT lineage (W1). By contrast, mutant fitness (M1 and M5) is dramatically lower than the WT, dropping to <1% even when starting from as high as approximately 73% (M1) or 83% (M5). b The schematic of our experimental setup. An ancestral WT colony was picked and plated to obtain individual colonies. One mutant and one WT colony were picked and plated to obtain six WT and six mutants clones. Lineages were subsequently transferred via single colony bottlenecks for 25 transfers. During each transfer the colonies were randomly-chosen and each time point of these transfers is designated as T plus the transfer number.
