Fig. 3: Fitness and antibiotic production in evolved lineages.
From: Mutational meltdown of putative microbial altruists in Streptomyces coelicolor colonies
a The fitness in terms of CFU of each lineage through time. WT lineages are shown in black with the one lineage W3 distinguished by a dashed line to indicate a conversion to mutant form after the 7th transfer. The mutant lineages all show an overall decline through time with one lineage, M2, completely dying after the 5th transfer. b Relative fitness of ancestor and evolved strains during competition with the WT. WT lineages show no significant change over time except for W1 and W3. The evolved strains in the mutant lineages show an overall decline in relative fitness except for M5. c Antibiotic production measured as the zone of inhibition for the ancestor and evolved strains of all lineages. WT lineages except for W3 show no change in antibiotic production. Mutant lineages show significant differences in antibiotic production. Dots and error bars represent means and 95% confidence intervals calculated from n = 2–4 biologically independent replicates (details are given in Methods). Levels of statistical significance are indicated as *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 (Welch’s t test or one-sample t test).
