Fig. 2: S. cerevisiae–SynJEC chimeras have a partially rescued respiration-competent phenotype.
From: Engineering artificial photosynthetic life-forms through endosymbiosis
a Release of ATP by SynJEC1 cells expressing the UWE25 ADP/ATP translocase in the presence of 80 μM ADP in comparison to SynJEC0 cells. ATP was released when SynJEC1 (expressing the ATP/ADP translocase) and SynJEC0 cells were challenged with extracellular ADP (80 μM), but not with a blank solution lacking ADP (N = 3 biological replicates; data are presented as mean values + /− SEM). Two-sided t-tests were used to compare means without adjustments (95% CI, Cohen’s d = 10.6, DF = 4, P = 0.0002; 95% CI, Cohen’s d = 13.0, DF = 4, P = 0.0001; 95% CI, Cohen’s d = 2.7, DF = 4, P = 0.0002). b Growth of S. cerevisiae cox2-60–SynJEC chimeras on medium containing glycerol as the sole carbon source. No growth was observed in round 4 for yeast lacking intracellular SynJEC. The experiment was repeated independently six times with similar results. c Total DNA isolated from spots grown on selection medium III contain the yeast-encoded MATa gene and SynJEC-encoded chloramphenicol acetyltransferase (CAT) gene. The experiment was repeated independently six times with similar results. d Growth rate of yeast-SynJEC chimeras on Selection Medium III. Cells (3.00 × 103) were spotted on Selection Medium III and counted after 72 h growth. (N = 3 technical replicates; data are presented as mean values+/− SEM.)P-values were calculated by two-tailed t-test comparing the two means. e Panel 1 describes the growth of S. cerevisiae-cox2-60 and S. cerevisiae-cox2-60-pCOX2n under non-selection conditions. Panel 2 describes the growth of S. cerevisiae-cox2-60 and S. cerevisiae-cox2-60-pCOX2n under selection conditions where the rescue in the growth of S. cerevisiae-cox2-60-pCOX2n is observed but no growth is observed for S. cerevisiae-cox2-60. The experiment was repeated independently three times with similar results. Source data are provided as a Source Data file.
