Fig. 3: Rescue of respiration deficient phenotype by yeast-SynJEC chimeras expressing an ATP/ADP translocase and SNARE-like proteins.
From: Engineering artificial photosynthetic life-forms through endosymbiosis
a Growth of S. cerevisiae cox2-60–SynJEC2—4 chimeras on medium containing glycerol as the sole carbon source. The experiment was repeated three times independently with similar results. b Total DNA of yeast-SynJEC chimeras contains yeast MATa and SynJEC CAT genes. The experiment was repeated three times independently with similar results. c, d Growth trends of S. cerevisiae cox2-60 (yeast only strain), S. cerevisiae cox2-60- SynJEC2 (yeast-SynJEC2), S. cerevisiae cox2-60- SynJEC3 (yeast-SynJEC3) and S. cerevisiae cox2-60- SynJEC4 (yeast-SynJEC4) chimeras on Selection Medium III. Cells (3.00 × 103) were spotted on Selection Medium III the final number of cells/spot on plate were determined after 72 h of growth (N = 3 technical replicates; data are presented as mean values+/− SEM). P-values were calculated by two-tailed t-test comparing the two means. Source data are provided as a Source Data file.
