Fig. 5: Rescue of respiration-deficient phenotype by yeast-SynJEC chimeras containing auxotrophic SynJEC5 cells.

a Growth of SynJEC5 cells in BG-11 medium in the presence and absence of L-methionine b Growth of yeast-SynJEC3 and yeast-SynJEC5 chimeras on Selection Medium III. The experiment was repeated three times independently with similar results. c Total DNA analysis of SynJEC5 DNA reveals the presence of genomic SNARE-like proteins Ctr-incA and CT813 (left) and disruption of the metA gene with a kanamycin resistance marker (right). The experiment was repeated twice independently with similar results. d Total DNA of yeast-SynJEC chimeras contains yeast MATa and SynJEC Ctr-incA genes. The experiment was repeated three times independently with similar results. e S. cerevisiae cox2-60-SynJEC3 chimeras are generated under and selected under photosynthetic conditions. After two rounds of growth S. cerevisiae cox2-60-SynJEC3 chimeras are propagated either under standard photosynthetic conditions or under dark; initial number of cells plated per spot is 10⁴ (N = 3 technical replicates; data are presented as mean values+/− SEM). P-values were calculated by two-tailed t-test comparing the two means (95% CI, Cohen’s d = 6.2, P = 0.0016). f SynJEC3 are starved for light for 72 h and the fused to S. cerevisiae cox2-60 and selected under dark conditions (dark fusions). The S. cerevisiae cox2-60-SynJEC3 chimeras generated from dark fusions fail to grow whereas the control S. cerevisiae cox2-60-SynJEC3 chimeras propagated under photosynthetic conditions propagate as previously observed. The experiment was repeated twice independently with similar results. g Plate images to demonstrate the effect of (3-(3,4-dichlorophenyl)−1,1-dimethylurea), DCMU, on S. cerevisiae cox2-60-SynJEC3 chimeras. The experiment was repeated twice independently with similar results. h Cell count analysis to demonstrate the effect of (3-(3,4-dichlorophenyl)−1,1-dimethylurea), DCMU, on S. cerevisiae cox2-60-SynJEC3 chimeras (N = 3 technical replicates; data are presented as mean values+/− SEM). P-values were calculated by two-tailed t-test comparing the two means. Source data are provided as a Source Data file.