Table 1 List of genes whose different degree of silencing consistently led to the typical Mit phenotypes.
Cosmid ID | Gene | Human ortholog, short gene description | Disease | RNAi Power | Phenotype |
|---|---|---|---|---|---|
ZK973.10 | lpd-5 | NDUFS4, NADH-Dehydrogenase, Fe-S protein complex I subunit | Complex I deficiency (Leigh Syndrome) | Mild Strong | Few progeny Slow development; few eggs |
Y45G12B.1 | nuo-5 | NDUFS1, NADH-Ubiquinone Oxidoreductase Fe-S Protein 1; complex I | Complex I deficiency (Leigh Syndrome) | Mild Strong | Pale, small, few eggs Slow development, arrested L2/L3 |
C09H10.3 | nuo-1* | NDUFV1, mitochondrial NADH Dehydrogenase Ubiquinone Flavoprotein 1 | Complex I deficiency (Leigh Syndrome) | Mild Strong | Pale, thin, sterile Arrested as L2/L3 |
T20H4.5 | T20H4.5* | NDUFS8, ubiquinone oxidoreductase core subunit S8 | Complex I deficiency (Leigh Syndrome) | Mild Strong | Pale, small, few eggs Arrested L2/L3 |
F53F4.10 | F53F4.10 | NDUFV2, Fe-S complex I | Complex I deficiency Parkinson’s Disease Susceptibility | Mild Strong | Slow development Slow development, arrested L2/L3 |
C01F1.2 | sco-1 | SCO-1, SCO cytochrome c oxidase assembly protein 1 | Cytochrome-c oxidase deficiency disease | Mild Strong | Pale, few progeny Slow development, sick, thin, sterile |
H14A12.2 | fum-1 | FH, fumarate hydratase, predicted to have fumarate hydratase activity | Fumarase deficiency, Leigh syndrome | Mild Strong | Few eggs Few progeny, slow development |
T12E12.4 | drp-1 | DNM1L (dynamin 1 like) | Optic dystrophy | Mild Strong | Similar to control Small, pale |
D2013.5 | eat-3* | OPA-1 or mgm-1, dynamin family GTPase | Dominant optic atrophy | Mild Strong | Thin Thin, slow development |
F54H12.1 | aco-2 | ACO2, Mitochondrial aconitase | Infantile cerebellar-retinal degeneration and optic atrophy 9 | Mild Strong | Few progeny Sterile |
Y47G6A.10 | spg-7* | AFG3L2, AFG3 like matrix AAA peptidase subunit 2 | Spinocerebellar ataxia (SCA28) | Mild Strong | Pale, small, thin Slow development, arrested L2 |
F23B12.5 | dlat-1* | DLAT, dihydrolipoamide S-acetyltransferase | pyruvate decarboxylase deficiency | Mild Strong | Sterile, slow moving Sterile |
K08E3.7 | pdr-1* | PARK-2 or parkin, E3 ubiquitin ligase | Parkinson’s disease | Mild Strong | Slow moving, pale, few progeny Slow moving, pale, few eggs, protruding vulva |
F25B4.6 | hmgs-1* | HMGCS1 & HMGCS2, 3-hydroxy-3-methylglutaryl-CoA synthase 1 & 2 | Mitochondrial HMG-CoA synthase deficiency | Mild Strong | Pale, thin, slow moving Sick, pale, slow moving |
C15F1.7 | sod-1 | SOD-1, Cu-Zn SOD1 | Amyotrophic lateral sclerosis | Mild Strong | Small Similar to control |
T22B11.5 | ogdh-1 | OGDH, predicted to have oxoglutarate dehydrogenase (succinyl-transferring) activity | Alpha ketoglutarate deficiency | Mild Strong | Thin, pale, sterile adults Thin, pale, slow development, sick |
W02F12.5 | dlst-1* | DLST, dihydrolipoamide S-succinyl transferase | Possible cause of familial Alzheimer’s disease | Mild Strong | Skinny, pale Long, pale |
Y46G5A.2 | cox-10 | COX10, complex IV farnesyl transferase | Mitochondrial complex IV deficiency | Mild Strong | Few eggs, pale Pale, few eggs |
T27E9.1 | tag-61* | SLC25A5, solute carrier family 25 member 5, mitochondrial adenine nucleotide transporters | Mitochondrial phosphate carrier deficiency | Mild Strong | Thin, pale Small, pale, sterile, protruding vulva |
F101.12 | aldo-2 | ALDOB, encodes a fructose-bisphosphate aldolase | Hereditary fructose intolerance | Mild Strong | Pale Sick, pale |
