Fig. 3: Callosal neurons display changes in spine morphology following TBI. | Nature Communications

Fig. 3: Callosal neurons display changes in spine morphology following TBI.

From: Selective plasticity of callosal neurons in the adult contralesional cortex following murine traumatic brain injury

Fig. 3

a Schematic representation of spine morphology and confocal images of mushroom, thin and stubby spines. Yellow arrows represent the examples of the respective spine type. Scale bar: 5 μm. b Scheme of layer II/III callosal cortical neuron and quantification (mean ± SEM) of the fraction of mushroom, thin and stubby spines on apical tuft and proximal dendrites of layer II/III callosal neurons. n = 6–9 independent dendritic stretches from 4 to 5 mice for Apical and n = 7–10 independent dendritic stretches from 4 to 5 mice for Proximal. Apical mushroom spines: p = 0.0040 Ctrl vs7d and p = 0.0102 Ctrl vs 14d. One way-ANOVA and Dunnett’s test. c Scheme of layer V callosal cortical neuron and quantification (mean ± SEM) of the fraction of mushroom, thin and stubby spines on apical tuft, distal and proximal dendrites of layer V callosal neurons. n = 7–10 independent dendritic stretches from 4 to 5 mice for Apical, n = 7–10 independent dendritic stretches from 4 to 5 mice for Distal, n = 6–10 independent dendritic stretches from 4 to 5 mice for Proximal. Apical mushroom spines: p = 0.0271 Ctrl vs7d and p = 0.0015 Ctrl vs 14d. One way-Anova and Dunnett’s test. Apical stubby spines: p = 0.0026 Ctrl vs 14d. One way-Anova and Dunnett’s test. d Scheme of layer II/III non callosal cortical neuron and quantification (mean ± SEM) of the fraction of mushroom, thin and stubby spines on apical tuft and proximal dendrites of layer II/III non callosal neurons. n = 5–6 independent dendritic stretches from 4 to 5 mice for Apical and n = 6–7 independent dendritic stretches from 4 to 5 mice for Proximal. e Scheme of layer V non callosal cortical neuron and quantification (mean ± SEM) of the fraction of mushroom, thin and stubby spines on apical tuft, distal and proximal dendrites of layer V non callosal neurons. (light gray: mushroom spines, dark gray: stubby spines, medium gray: thin spines). n = 6–12 independent dendritic stretches from 4 to 5 mice for Apical, n = 7–13 independent dendritic stretches from 4 to 5 mice for Distal, n = 8–12 independent dendritic stretches from 4 to 5 mice for Proximal. Statistical p values are always compared to control (Ctrl) values. Source data are provided as a Source Data file.

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