Fig. 5: Alternative fates of Cit-k KO dorsal vs. ventral OPCs are due to cell-intrinsic factors and associated with their diverse developmental origin. | Nature Communications

Fig. 5: Alternative fates of Cit-k KO dorsal vs. ventral OPCs are due to cell-intrinsic factors and associated with their diverse developmental origin.

From: Molecular and functional heterogeneity in dorsal and ventral oligodendrocyte progenitor cells of the mouse forebrain in response to DNA damage

Fig. 5

a NG2+ (red) cells in distinct regions of Sox10Cre (Ctrl) vs. Sox10Cre;Cit-kfl/fl mouse forebrain at P14. b NG2+ cell density in the dorsal cortex, corpus callosum, striatum and preoptica area at P14 (Sox10Cre n = 4; Sox10Cre;Cit-kfl/fl n = 5. Two-tailed Unpaired t-test, Dors. Ctx: P = 0.0073, t(7) = 3.741; CC: P = 0.0038, t(7) = 4.249; Str: n.s.; POA: n.s.). c Percentage of cCASP3+ OPCs in distinct regions of P14 Sox10Cre (Ctrl; n = 3) vs. Sox10Cre;Cit-kfl/fl (n = 5) mice (Chi-Square Test, Dors.Ctx/CC: P < 0.0001, χ2(1) = 15.98). (inset) Representative PDGFRα+(green)/cCASP3+(red) OPC in Sox10Cre;Cit-kfl/fl mouse cortex. d SA-βGAL+(blue)/Sox10+(brown) oligodendroglial cells in Sox10Cre;Cit-kfl/fl mouse striatum. e Expression of mRNAs coding for apoptosis- and senescence- associated proteins in dOPCs vs. vOPCs MACSorted from P10 Sox10Cre;Cit-kfl/fl mice (Two-tailed Paired t-test, n = 4/group; Bbc3, n.s.; p21, P = 0.049, t(3) = 3.204; Ecrg4: P = 0.011, t(3) = 5.744). f NG2+ (red) cells in the CC and deep layers of the dorsal cortex of Emx1Cre;R26RYFP (Ctrl) vs. Emx1Cre;R26RYFP;Cit-kfl/fl P14 mice. YFP (green)-positivity identifies cells derived from dorsal Emx1+ progenitors. g NG2+ (red) cells in the preoptic area of Nkx2.1Cre;R26RYFP (Ctrl) vs. Nkx2.1Cre;R26RYFP;Cit-kfl/fl P14 mice. YFP (green)-positivity identifies cells derived from ventral Nkx2.1+ progenitors. h NG2+ and NG2+/YFP+cell density in the dorsal cortex and corpus callosum of P14-P16 Emx1Cre;R26RYFP (Ctrl, n = 4) vs. Emx1Cre;R26RYFP;Cit-kfl/fl (n = 4. Two-tailed Mann–Whitney U-test, Dors. Ctx: tot: n.s.; YFP + : 0.028; CC: tot: n.s.; YFP + : 0.028). i Percentage of cCASP3+ OPCs in Dors.Ctx/CC of P14-P16 Emx1Cre;R26RYFP (Ctrl, n = 3) vs. Emx1Cre;R26RYFP;Cit-kfl/fl (n = 4) mice (Chi-Square Test: Dors.Ctx/CC: P = 0.0038, χ2(1) = 8.362). j NG2+ and NG2+/YFP+cell density in the preoptica area of P14-P16 Nkx2.1Cre;R26RYFP (Ctrl, n = 4) vs. Nkx2.1Cre;R26RYFP;Cit-kfl/fl (n = 4. Two-tailed Mann–Whitney U-test, tot: n.s.; YFP + : n.s.). Data in b, c, h, i, j are mean ± SE. In e lines connect paired samples (i.e., dOPC and vOPC samples from the same mouse). DAPI (blue) in a, f, g counterstains cell nuclei. Scale bars: 30 µm in a, f, g; 10 µm in d. CC corpus callosum, Ctrl control, Dors. Ctx. dorsal cortex, POA preoptica area, P postnatal day. ***P < 0.001; **P < 0.01, *P < 0.05. Source data are provided as a Source Data file.

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