Fig. 5: Polyubiquitination is required for SERINC5 downregulation by HIV-1 Nef. | Nature Communications

Fig. 5: Polyubiquitination is required for SERINC5 downregulation by HIV-1 Nef.

From: Cul3-KLHL20 E3 ubiquitin ligase plays a key role in the arms race between HIV-1 Nef and host SERINC5 restriction

Fig. 5

A Ser5 and its lysine mutants were expressed with HIV-1 Nef in HEK293T cells, and their expression was analyzed by WB. Ctrl, control vector. B Ser5 and its lysine mutants were expressed with Ub and Nef in HEK293T cells, and their expression was analyzed by WB. C Ser5-VN and its lysine mutants were expressed with Nef-VC in HeLa cells. Cells were stained with DAPI and anti-HA for Ser5, and BiFC fluorescent signals were detected by confocal microscopy (scale bar 2 or 5 µm). Experiments were repeated twice, and representative experiments are shown. Source data are provided as a Source Data file.

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