Fig. 3: Molecular recognition of PACAP27 by VIP2R.
From: A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2
a The binding mode of PACAP27 (gold) with VIP2R (light green), showing that the N-terminal half of PACAP27 penetrates into a pocket formed by TMs 1-3, TMs 5-7, and ECLs 1-3, whereas the C-terminal half is recognized by the ECD, ECL1, and TM1. The ECD and ECL1 are shown as surface. b–e Close-up views of the interactions between PACAP27 and VIP2R. Key residues are shown as sticks. Concentration-response curves of VIP2R mutants that show <3-fold effect (dark gray), 3- to 15-fold effect (sky blue), 15- to 100-fold effect (salmon), or >100-fold effect (plum). f Signaling profiles of VIP2R mutants. cAMP accumulation in wild-type (WT) and single-point mutated VIP2R expressing CHO-K1 cells. Signals were normalized to the maximum response of the WT and concentration-response curves were analyzed using a three-parameter logistic equation. All data were generated and graphed as means ± S.E.M. of at least three independent experiments, conducted in quadruplicate (n = 3–6). Source data are provided as a Source Data file.
