Fig. 1: Actin stability declines with age.

a Representative SIM images of F-actin and bud scars of young and old wild-type (WT) cells stained with Alexa488-phalloidin and Alexa594-wheat germ agglutinin (WGA594), respectively. Yellow arrow identifies a depolarized actin cable. b Quantification of Alexa488-phalloidin corrected mean signal intensity in mother cells of young and old cells isolated from mCAD. Combined n of cells/strain from 3 trials: 248 (young) and 152 (old). The p value (<1.00e⁻¹⁵) was determined by a two-tailed non-parametric Mann–Whitney test. c Representative SIM images of Alexa488-phalloidin-stained actin and Alexa594-WGA-stained bud scars in young and old WT cells treated with vehicle (DMSO) or 1 µM Lat-A for 10 mins. d Time-course of actin cable loss during treatment with 1 µM Lat-A. Greater than 126 cells/timepoint/condition/trial were analyzed for n of 3 trials. Error bars: SEM. e Growth curves of deletion strains that exhibit reduced sensitivity to Lat-A (10 µM) in our genome-wide screen. f Actin cable abundance in WT cells and the 13 hits from the screen. Actin was visualized by rhodamine-phalloidin staining and wide-field fluorescence microscopy. Error bars: SEM. Scale bars, 2 µm.