Fig. 2: Identification of a gene (YKL075C) that affects actin cable stability, mitochondria, and lifespan.

a Growth curves of WT and ykl075c∆ cells treated with vehicle (DMSO) or 2 µM Lat-A for 16 h. Data from a representative trial is shown (n = 3 trials). b Representative images of Alexa488-phalloidin stained F-actin of WT and ykl075c∆ cells. c Actin cable abundance in mother cells of mid-log phase WT and ykl075c∆ cells. n = 44 cells (WT) and 26 cells (ykl075c∆). Data from a representative trial is shown (n = 3 trials). p value (1.00e⁻⁰⁶) was determined by a two-tailed non-parametric Mann-Whitney test. d Representative images of Alexa488-phalloidin stained F-actin of WT and ykl075c∆ cells treated with vehicle (DMSO) (top panels) or 2 µM Lat-A for 20 min (bottom panels). e Quantitation of actin cables in WT and ykl075c∆ cells during treatment with 2 µM Lat-A. Greater than 256 cells/strain/timepoint were analyzed for n = 5 trials. Error bars: SEM. p value (8.8e⁻⁰³) was determined by simple linear regression analysis. f RLS of WT and ykl075c∆ cells determined by manual micromanipulation. n of cells/strain: 35 (WT) and 36 (ykl075c∆). p value (1.23e⁻⁰⁶) was determined by the two-sided Mantel-Cox test. Data from a representative trial from n of 3 trials. g Representative images of mitochondrial redox state (reduced:oxidized ratio) visualized using mdito-roGFP1 in WT and ykl075c∆ cells. Higher numbers/warmer colors indicate more reducing mitochondria. h Quantification of mito-roGFP1 redox ratios in WT and ykl075c∆ cells. Combined n of cells/strain from three trials: 284 (WT) and 260 (ykl075c∆). p value (< 1.00e⁻¹⁵) was determined by a two-tailed non-parametric Mann–Whitney test. Scale bars, 2 µm.