Fig. 3: Anti-vimentin antibodies inhibit angiogenesis.
From: Extracellular vimentin mimics VEGF and is a target for anti-angiogenic immunotherapy
a HUVEC scratch wound analysis in the presence of anti-vimentin antibodies (Vim Ab). n = 4 different donors. Data represent means ± SEM. p values represent two-way ANOVA with Dunnett’s correction for multiple comparisons for treatment. Representative images are shown in the right panel. b, c Tube formation of HUVEC on Matrigel in the presence of anti-vimentin antibodies (Vim Ab) or control antibodies (Ctrl Ab) n = 4 different donors. Bar graphs represent means ± SEM. p values represent unpaired t test. Representative images are shown. d, e Vessel density in physiological CAMs (d) and after photodynamic therapy (PDT) (e), treated with Vim Ab or Ctrl Ab. n = 3 (d), and n = 10 (Ctrl Ab) n = 11 (Vim Ab) (e) eggs/group. Bar graphs represent means ± SEM. p values represent unpaired t test. Representative images are shown to the right of the graphs. f Fluorescently labeled Vim Ab after i.v. injection localizes to the tumor vasculature in the CAM spheroid (arrow). Bottom panel: magnification of white box. Representative images of a single experiment are shown. g–i HCT116 xenograft tumor growth on the CAM, topically treated daily with 100 µl antibody or 2 µM sunitinib. g Tumor growth. n = 8 (Vim Ab), n = 9 (Ctrl, sunitinib) eggs/group. Data represent means ± SEM. p values represent two-way ANOVA with Dunnett’s correction for multiple comparisons for treatment. h Microvessel density (MVD) in Vim Ab (n = 7) and Ctrl (n = 6) treated tumors on the CAM. Data represent means ± SEM. p values unpaired t test. i Detection of tumor-homed antibodies in n = 12 (Ctrl Ab) and n = 14 (Vim Ab) images/group. Representative images are shown. j Passive Vim Ab therapy of B16F10 melanoma tumor growth in mice. n = 10 mice/group, p values represent two-way ANOVA. k MVD in n = 3 fields/tumor for n = 3 mice/group. Data represent means ± SEM. p values represent one-way ANOVA with Bonferroni correction. l Tissue distribution of 89-Zr labeled anti-vimentin nanobodies in mice (n = 2) with B16F10 melanoma (T = tumor, K = kidney, L = liver). Data represent means ± SEM. Source data are provided as a Source Data file.
