Table 2 Sites of fluorophore incorporation within fluorescent Stop-POST5 complexes.

From: Ataluren binds to multiple protein synthesis apparatus sites and competitively inhibits release factor-dependent termination

Stop-POST5

Lys within FKVRN

tRNAGln

Ribosomea

Atto(pep)

Atto647

unlabeled

unlabeled

Atto(pep)-Cy(tRNA)

Atto647

Cy3

unlabeled

Atto(rbsm)-Cy(tRNA)

unlabeled

Cy3

Atto647N

Atto(pep)-Cy(rbsm)

Atto647

unlabeled

Cy3

  1. aribosomes were labeled to a total stoichiometry of ~1/ribosome spread over Lys residues within r-proteins
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