Fig. 2: LC-HRMS analysis of DarApk produced in vivo, showing the extracted ion chromatograms (EICs) and MS spectra.
From: Radical SAM-dependent ether crosslink in daropeptide biosynthesis
a DarApk produced by coexpression with DarEpk carries a + 12 Da modification. Because heterologous expression in E. coli only produced obtained the truncated DarApk, the theoretically predicted signals corresponding to the unmodified DarApk were shown for comparison. See Supplementary Fig. 3 for the HR-MS/MS spectrum of DarApk(43-58)-1. b DarApk produced by coexpression with the C86A mutant of DarEpk is a truncated peptide (i.e., DarApk(1-48)), which lacks the core and follower regions.
