Fig. 2: Dynamic heterogeneity of the Aundiff population during initiation of regeneration.
From: Distinctive molecular features of regenerative stem cells in the damaged male germline
a Visualisation of single-cell RNA-Seq data from Aundiff (E-Cadherin + α6-integrin+ c-KIT–) isolated from CTRL and BU mice at D10 by UMAP. Bar chart illustrates distribution of Aundiff from CTRL and BU-treated samples into different cell clusters and identity of each cluster. b Violin plots comparing expression of selected markers associated with stem and progenitor populations in cell clusters from a. Cell cycle-associated genes that delineate cell clusters are shown in bottom panels. c Heatmap of top 10 DEGs in cell clusters identified from analysis of a. d GO terms associated with gene expression profiles of cell clusters identified in a. GO terms enrichment identified by one-sided Fisher’s exact test with Benjamini–Hochberg correction for multiple comparisons. e SCENIC analysis identifying cluster-enriched regulons of control samples from a. Heatmap of top ten regulons that define each cluster or cell state are shown and selected regulons highlighted. Number of genes (g) in each regulon is indicated. f scVelo plot embedding in the UMAP space reveals directional progression of transcriptional states.
