Fig. 5: SARS-CoV-2 infection induces DAXX cytoplasmic re-localization to sites of viral replication.

DAXX overexpression restricts SARS-CoV-2. 293T-ACE2 cells were transfected with DAXX WT. 24 h after transfection, cells were infected with the mNeonGreen fluorescent reporter SARS-CoV-2 at the indicated MOI. Cells were either visualized with an EVOS fluorescence microscope (a, b) or stained with an HA-antibody detecting DAXX and imaged by confocal microscopy (c). Scale bars correspond to 200 µm (a) and 30 µm (c). Images shown in (a) were quantified using ImageJ software (b). Data shows the mean ± SD of Fluorescence integrated densities. The analysis was performed on around 200 cells from 3 different fields. Images are representative of 3–6 different fields from 2 independent experiments. d Relocalization of endogenous DAXX during SARS-CoV-2 infection. 293T-ACE2 cells were infected with SARS-CoV-2 at an MOI of  1. 24 h post-infection, cells were labelled with Hoescht and with antibodies against dsRNA (detecting viral RNA, in green) and HA (detecting DAXX, in red). When indicated, the high-resolution Airyscan mode was used. Scale bars correspond to 10 µm for confocal images, and 2 µm for the high-resolution images. Images are representative of 3–6 different fields from 2 independent experiments. Source data are provided as a Source Data file.