Fig. 1: Sequencing RNA of single phase-separated coacervates.

a Schematic of coacervate generation and single-coacervate sequencing strategy. Coacervates were generated mixing charged polyelectrolytes Carboxymethyldextran with PDDA (CM-Dex:PDDA). Scale bar = 100 µm. Total RNA isolated from iPS was used as RNA input. Single coacervates were sorted into 96-well plates using fluorescence-activated cell sorting (FACS). RNA was extracted from each coacervate and mRNA was converted to cDNA and sequenced upon library preparation. RNAs present in each sequenced coacervate were computationally identified and quantified. b Schematic illustration of cross comparisons of several parameters (RNA length, coacervate size and complexity of RNA pool) from hundreds of individual coacervates in a single assay. c Relationship between the size of single coacervates, the number of different RNA transcripts and the average length of all RNA transcripts in each coacervate. Each dot represents a sequenced coacervate. Coacervate size was measured by the FACS forward scatter (FSC).