Fig. 3: Properties of RNA found within coacervates.

a Correlation between input RNA amount and the frequency with which each transcript is detected in CM-Dex:PDDA coacervates. Transcripts that are enriched in coacervates (defined as residuals >30 for generalized additive model) are labeled red. b Analysis of sequence motifs that are detected within enriched transcripts (as defined in a) or randomly selected non-enriched transcripts. Motif enrichment corresponds to E-value derived from MEME suite. Among enriched transcripts, the two most abundant sequence motifs (Motifs 1 and 2) display sequence complementarity. c Frequency of transcript detection in coacervates conditional on if the transcripts contain either Motif 1, Motif 2, both motifs or none. d Analysis of sequence complementarity among different transcripts present in the pool of enriched or randomly selected transcripts. Sequence complementarity was determined using local-pairwise alignment (Smith–Waterman) scores. Dotted line indicates the maximum complementarity score that was detected outside the enriched vs. enriched comparisons (gray bars). e Comparison of sequence similarity of enriched motifs to known genomic elements. Heatmap represents pairwise alignment (Smith–Waterman) of enriched motifs with sequences of short interspersed elements (SINEs). Color intensity represents alignment score.