Fig. 1: SUN proteins interact with POD1.
From: POD1-SUN-CRT3 chaperone complex guards the ER sorting of LRR receptor kinases in Arabidopsis
a Phylogenetic tree of SUN proteins. b Domain structure of AtSUN1/2 and AtSUN3/4/5. c–e SUN3, 4, and 5 interact with POD1 in Arabidopsis protoplasts by Co-IP, but not with ER chaperone CNX1. f SUN4-GFP is detected in the microsome fractions (P100) after cellular fractionation . PDIL1-5 and CNX1 were used as the control of soluble (S100) and membrane protein (P100), respectively. T, total protein. g SUN3 and SUN4 are N-Âglycosylated proteins. Total proteins extracted from protoplasts expressing SUN3-HA and SUN4-GFP, respectively, were treated with (+) or without (-) N-Âglycosidases PNGase F and probed for the GFP tag. h Transcripts of SUN3, SUN4, and SUN5 were detected in inflorescences, mature flowers, pollen at stage 12c and mature pollen by RT-PCR. i Schematic diagrams of the gene structures of SUN3, SUN4, and SUN5, and the T-DNA insertion sites of mutants. Dark grey, exons; light grey, UTRs; lines, introns; triangle, the T-DNA insertion site. j The transcripts of SUN3/4/5 were not detected in sun3/4/5 triple mutant by RT-PCR. 3 (c–h) and  5 (j) independent biological experiments were repeated.
