Fig. 5: Localization of cargoes in sun3/4/5 pollen tubes.
From: POD1-SUN-CRT3 chaperone complex guards the ER sorting of LRR receptor kinases in Arabidopsis
a Plasma membrane (pm) localization of PRK2-GFP in the WT. b PRK2-GFP enriched intracellular vesicles (iv) were stained with LysoTracker red in sun3/4/5. c PRK3-GFP is localized on the PM and intracellular vesicles in the WT. d The PM localization of PRK3 is impaired in sun3/4/5, and mainly localized in acidic vesicles stained by LysoTracker red. e The PM localization of MDIS1 was partially impaired in sun3/4/5. f Wortmannin (Wm) treatment disrupts the PM localization of PRK2-GFP at the apex and subapex region of WT pollen tubes. PRK2-GFP signal disappeared after Wm treatment in sun3/4/5. g In sun3/4/5, PRK3-GFP signal disappeared after Wm treatment, but in WT, the signal on the PM and vesicles remained. h The PM localization of GFP-Lit6b in sun3/4/5. i The PM localization of GFP-Lit6b after Wm treatment in sun3/4/5. j The vesicle localization of RabA4d at the apex region is not affected in sun3/4/5. k The PM localization of GFP-Sec3A is not affected in sun3/4/5. 3 (a-k) independent biological experiments were repeated. Bar, 5 μm. v, vacuole.
