Fig. 2: Performance of combinatorial systems at the single-gene knockout level.

a–c The single-gene receiver operating characteristic (ROC)—area under the curve (AUC) curves derived from pan-essential and nonessential genes across distinct libraries in (a) IPC298, (b) MELJUSO, and (c) PK1. AUC values are indicated in parentheses. d Separation between gene scores for common essential genes and nonessential genes computed using null-normalized median difference (NNMD). e Correlation between the average LFC of right versus left sgRNAs coupled with sgAAVS1 cutting control in IPC298. Pearson’s correlation coefficient is indicated (r value) in black. Percentage of pan-essential genes with LFC less than −1 for sgRNAs in both right and left positions are indicated in red. Pan-essential genes are annotated by red dots. Source data are provided in the Source Data file.