Fig. 3: SARS-CoV-2 HexaPro-bearing immunogens induced a potent humoral immune response against SARS-CoV-2 prototype and circulating variants in cynomolgus macaques.
a Summary of ELISA binding (b), pseudovirus (c), and authentic virus (d) neutralizing antibody titers. The binding, pseudovirus and authentic virus neutralization antibody titers of HexaPro-bearing immunogen-elicited sera from cynomolgus macaques following each vaccination 4 weeks apart present as a heatmap colored in red, orange and green, respectively. A deeper red, orange and green represents a higher binding antibody titer, pseudovirus neutralization antibody titer and authentic virus neutralization antibody titer of sera from HexaPro-bearing immunogens-immunized mice, respectively. b HexaPro-specific endpoint binding titers of HexaPro-based immunogen-elicited sera in cynomolgus macaques following each vaccination 4 weeks apart against wild-type SARS-CoV-2 and variants measured by ELISA. c Pseudovirus neutralization titers of HexaPro-based immunogen-elicited sera from cynomolgus macaques following each vaccination 4 weeks apart against wild-type SARS-CoV-2 and a panel of 5 variants. ID50, reciprocal serum half-maximal neutralization in the pseudovirus-based neutralization assay. d Authentic virus neutralization titers of HexaPro-based immunogen-elicited sera from cynomolgus macaques following each vaccination 4 weeks apart against ancestral SARS-CoV-2 and a panel of 5 variant strains. NT50, reciprocal serum half-maximal neutralization in the cytopathic effect-based neutralization assay. In b–d, the dots indicate log10 (mean ± SD) titers. Each group contained four cynomolgus macaques. The statistical significance of each group was compared and analyzed using the two-tailed Mann–Whitney U test. Source data are provided as a Source Data file.
