Fig. 5: mGSDMA3Nterm oligomers reshape into more stable assemblies.
From: Gasdermin-A3 pore formation propagates along variable pathways
a–d All-atom MD simulations of membrane-inserted ring-shaped oligomers encompassing 18, 21, 27 and 30 mGSDMA3Nterm and reshaping after indicated times. Views are from cytosolic surface. E. coli polar lipid extract membranes are shown in gray, mGSDMA3Nterm in purple. e Topograph of membrane-inserted ring-shaped oligomer of 30 mGSDMA3Nterm averaged from 4000 oligomers from one MD simulation after exclusion of the first 100 ns. f Correlation symmetrized average of AFM topographs of 18 membrane-inserted ring-shaped mGSDMA3Nterm oligomers showing 30-fold symmetry (Fig. 4d). g Ring-shaped oligomer without β-barrel disassembles in solution within 100 ns. Scale bars, 10 nm.
