Fig. 2: XIST RNA coating in female hESCs cultured in atmospheric vs. physiological O2 concentration.
From: Preventing erosion of X-chromosome inactivation in human embryonic stem cells
a–d Percentage of nuclei with XIST RNA coats per colony of hESC lines UM63-1 (a, c) and UM77-2 (b, d) cultured in parallel under 20% (a, b) and 5% (c, d) O2 concentration on Matrigel in mTeSR1 medium. The difference in the frequency of nuclei without XIST RNA coats per colony in either cell line when cultured at physiological vs. atmospheric O2 concentration is not significant (general linear model comparison, p = 0.1). See also Supplementary Fig. 2. At least 100 nuclei were counted per colony for hESC RNA FISH quantification. The total number of colonies quantified at each passage range between 11–93 and are cataloged in source data. Source data are provided as a Source Data file.
