Fig. 3: Impact of culture surface on XIST RNA coating in female hESCs.
From: Preventing erosion of X-chromosome inactivation in human embryonic stem cells
a, b Percentage of nuclei with XIST RNA coats in colonies of hESC line UM63-1 (a) and UM77-2 (b) cultured on HFFs in XF medium under 5% O2 concentration. The frequency of nuclei harboring XIST RNA coats per colony in either cell line when cultured on HFFs did not decrease significantly (general linear model, p = 0.09). At least 100 nuclei were counted per colony for hESC RNA FISH quantification. The total number of colonies quantified at each passage range between 1 and 126 and are cataloged in source data. Source data are provided as a Source Data file.
