Fig. 4: Impact of culture medium on XIST RNA coating in female hESCs.
From: Preventing erosion of X-chromosome inactivation in human embryonic stem cells
a–d Percentage of nuclei with XIST RNA coats per colony in hESC lines UM63-1 (a, c) and UM77-2 (b, d) cultured in parallel in XF medium (a, b) and mTeSR1 medium (c, d) on HFFs. hESCs cultured with mTeSR1 medium displayed a significant decrease in nuclei with XIST RNA coating compared to hESCs cultured in XF medium during passaging (general linear model comparison; p < 0.001). All hESCs in this experiment were cultured in 5% O2 on HFFs. The quantification data for P13-14 in b are taken from Fig. 3b. At least 100 nuclei were counted per colony for hESC RNA FISH quantification. The total number of colonies quantified at each passage range between 10 and 180 and are cataloged in source data. Source data are provided as a Source Data file.
