Fig. 5: Analysis of culture media switching on XIST RNA coating in female hESCs.
From: Preventing erosion of X-chromosome inactivation in human embryonic stem cells
Percentage of nuclei with XIST RNA coats per colony of hESC line UM63-1 (a) cultured continuously in XF medium and (b) cultured initially in XF medium and subsequently switched to mTeSR1 medium. Percentage of nuclei with XIST RNA coats per colony of hESC line UM63-1 (c) continuously cultured in mTeSR1 medium and (d) cultured initially in mTeSR1 medium and then switched to XF medium. hESCs cultured initially in XF medium and subsequently switched to mTeSR1 medium displayed a significant decrease in nuclei with XIST RNA coating during passaging compared to hESCs cultured continuously in XF medium (general linear model comparison, p = 0.01). hESCs cultured continuously in mTeSR1 medium displayed a significant decrease in nuclei with XIST RNA coating during passaging compared to hESCs cultured initially in mTeSR1 medium and then switched to XF medium (general linear model comparison, p < 0.001). All hESCs in this experiment were cultured in 5% O2 on HFFs. See also Supplementary Fig. 3 and Supplementary Fig. 4. At least 100 nuclei were counted per colony for hESC RNA FISH quantification. The total number of colonies quantified at each passage range between 2 and 148 and are cataloged in source data. Source data are provided as a Source Data file.
