Fig. 6: LiCl in mTeSR1 Medium as a Cause of XIST RNA Loss in Female hESCs.
From: Preventing erosion of X-chromosome inactivation in human embryonic stem cells
a–c Percentage of nuclei with XIST RNA coating in colonies of hESC line UM90-14 cultured in XF medium (a), XF medium supplemented with 0.98 mM LiCl (XF with LiCl) (b), and mTeSR1 medium (c). hESCs cultured in XF medium did not display a significant decrease in nuclei with XIST RNA coats across passaging (general linear model, p = 0.2). hESCs cultured in XF medium with LiCl and mTeSR1 medium lost XIST RNA coating in a significant percentage of nuclei per colony during passaging compared to cells cultured in XF medium (general linear model, p < 0.001). All hESCs in this experiment were cultured in 5% O2 on HFFs. At least 100 nuclei were counted per colony for hESC RNA FISH quantification. The total number of colonies quantified at each passage range between 5 and 78 and are cataloged in source data. Source data are provided as a Source Data file.
